Coherent Doppler wind lidar with real-time wind processing and low signal-to-noise ratio reconstruction based on a convolutional neural network

Multi-classification using a convolutional neural network (CNN) is proposed as a denoising method for coherent Doppler wind lidar (CDWL) data. The method is intended to enhance the usable range of a CDWL beyond the atmospheric boundary layer (ABL). The method is implemented and tested in an all-fiber pulsed CWDL system operating at 1550 nm wavelength with 20 kHz repetition rate, 300 ns pulse length and 180 µJ of laser energy. A real-time pre-processing using a field programmable gate array (FPGA) is implemented producing averaged lidar spectrograms. Real-world measurement data is labeled using conventional frequency estimators and mixed with simulated spectrograms for training of the CNN. First results of this methods show that the CNN outperforms conventional frequency estimations substantially in terms of maximum range and delivers reasonable output in very low signal-to-noise (SNR) situations while still delivering accurate results in the high-SNR regime. Comparing the CNN output with radiosonde data shows the feasibility of the proposed method

A Novel Single Frequency, Pulsed UV Source For Airborne Direct Wind Lidar

We have conceptualized a compact UV source with pulse parameters optimized for airborne clear air turbulence detection. An amplified single frequency ns-Nd:YAG source is frequency-tripled to 355 nm, > 2.5W at 3 kHz pulse repetition frequency

Precision and accuracy of single-molecule FRET measurements - a multi-laboratory benchmark study

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straightforward method, we obtained FRET efficiencies with s.d. between ±0.02 and ±0.05. We suggest experimental and computational procedures for converting FRET efficiencies into accurate distances, and discuss potential uncertainties in the experiment and the modeling. Our quantitative assessment of the reproducibility of intensity-based smFRET measurements and a unified correction procedure represents an important step toward the validation of distance networks, with the ultimate aim of achieving reliable structural models of biomolecular systems by smFRET-based hybrid methods

Reliability and accuracy of single-molecule FRET studies for characterization of structural dynamics and distances in proteins

Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study of biomolecular structure and dynamics in vitro and in vivo. We performed an international blind study involving 19 laboratories to assess the uncertainty of FRET experiments for proteins with respect to the measured FRET efficiency histograms, determination of distances, and the detection and quantification of structural dynamics. Using two protein systems with distinct conformational changes and dynamics, we obtained an uncertainty of the FRET efficiency ≤0.06, corresponding to an interdye distance precision of ≤2 Å and accuracy of ≤5 Å. We further discuss the limits for detecting fluctuations in this distance range and how to identify dye perturbations. Our work demonstrates the ability of smFRET experiments to simultaneously measure distances and avoid the averaging of conformational dynamics for realistic protein systems, highlighting its importance in the expanding toolbox of integrative structural biology